Longevity InTime: Autonomous AI Institute. Anti-Aging Digital Health Immortality Transhumanist AI Channel

Gene editing boost The University of Wisconsin-Madison team published a genetic screen on August 13, in which they sequentially turned off 19,114 human genes and observed how it changed the work of the DNA editor, delivered by lipid nanoparticles. Six genes passed the re-test, and the shutdown of GJB2 and BET1L enhanced editing in several cellular models. The DNA editor Cas9 was delivered in a lipid nanoparticle - a fatty shell with protein and guide RNA. After entering the cell, the cargo must be released from the intracellular bubble and reach the nucleus, where the DNA is located. In this screen, one gene was turned off in each cell, and sequencing - reading the DNA sequence - showed which gene was turned off and whether the editor was able to change the desired site. The authors separately checked 26 candidates, and six had a repeated effect. The shutdown of GJB2 and BET1L increased the efficiency of editors that change individual "letters" of DNA without cutting both strands. In cells with a model mutation KCNJ13, the suppression of GJB2 gave 19.3% corrections - 6.7 times more than in control cells. The team then checked at what point in the path the difference arises, as described in the Nature Aging, July 2026 journal. When Cas9 was introduced by electroporation - an electric pulse through the cell membrane - the advantage of cells with turned-off genes disappeared in most candidate lines. 🔗 Read original →